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5,59-dithiobis(2-nitrobenzoic acid) (DTNB) (6 mM). GSSG reductase (266
U/ml) was added to initiate the assay, and the change in absorbance was
read at 412 nm. GSSG was determined by the same reaction, except that
GSH was blocked from reacting with DTNB by a 1-h derivatization at
258C with 2-vinylpyridine. It was determined that GSH assay recording
remained linear down to 0.005 nmol/mg tissue and GSSG to 0.001 nmol/
mg tissue.
5,59-dithiobis(2-nitrobenzoic acid) (DTNB) (6 mM). GSSG reductase (266
U/ml) was added to initiate the assay, and the change in absorbance was
read at 412 nm. GSSG was determined by the same reaction, except that
GSH was blocked from reacting with DTNB by a 1-h derivatization at
258C with 2-vinylpyridine. It was determined that GSH assay recording
remained linear down to 0.005 nmol/mg tissue and GSSG to 0.001 nmol/
mg tissue.